Comparing Amplification-Based and Amplification-Free Metagenomic Next-Generation Sequencing for Infectious Disease Diagnosis: A Methodological Assessment for Optimal Clinical Application and Antibiotic Use.

https://pubmed.ncbi.nlm.nih.gov/38094744

This study suggests that while both amplification-based and amplification-free metagenomic next-generation sequencing (mNGS) tests show promise in diagnosing infectious diseases, they have specific advantages and disadvantages, and their clinical application requires further methodological exploration for optimal performance and accuracy in guiding antibiotic use.