“Sensitive and Specific Triplex Real-Time PCR Assay for Simultaneous Detection of H3, H4, and H5 Subtypes of Avian Influenza Virus in Poultry” (in sentence case) or “Development of a Sensitive and Specific Triplex Real-Time PCR Assay for Simultaneous Detection of H3, H4, and H5 Subtypes of Avian Influenza Virus in Poultry” (in title case) Note: I have assumed that the text is for a title of a research article or study, hence the suggested titles are fairly descriptive and specific to the content of the given text.