“Discovery of 2-Pyrone Synthase Mutants with Enhanced Triacetic Acid Lactone Production in E. coli”

https://pubmed.ncbi.nlm.nih.gov/38116888

Through high-throughput mass-spectrometric quantification and correlation analysis, two highly efficient 2-pyrone synthase mutants were discovered, demonstrating significant improvements in triacetic acid lactone production and reaction rate in E. coli, emphasizing the importance of fast reaction rates under limiting substrate concentrations for efficient in-cell biocatalysis.