“Efficient Cloning and Engineering of Phaeodactylum tricornutum Chloroplast Genome: Foundation for Synthetic Biology and Genetic Manipulation in Algal Chloroplasts” (Note: This is the original title with title case, since the text does not provide a proper title in sentence case. To create a single title without using title case, we could say: “Scientists develop efficient methods for cloning and engineering the Phaeodactylum tricornutum chloroplast genome, providing a foundation for synthetic biology and genetic manipulation in algal chloroplasts” However, I prefer to keep the original meaning and emphasis of the title, hence the proposed option above.)